Science Image Library - Mycobacterium Photos
Science Image Library
from Science Prof Online
You have free access to a large collection of materials used in a college-level introductory microbiology course. The Virtual Microbiology Classroom provides a wide range of free educational resources including PowerPoint Lectures, Study Guides, Review Questions and Practice Test Questions.
1 & 2. Streak plate of Mycobacterium smegmatis colonies growing on TSY agar, top view; 3. Streak plate of Mycobacterium smegmatis colonies growing on TSY agar, bottom view; 4 & 5. M. smeg written in the bacteria Mycobacteria smegmatis growing on TSY agar. Note, in close-up photo, the waxy appearance of the bacterial colonies.
Mycobacterium smegmatis Bacterial Colonies (Click on photo to enlarge.)
1 & 2: Acid-fast stain showing positive (Mycobacterium) results @ 1000xTM; 3 & 4. Mixed slides of acid-fast Mycobacterium (bright pink) and nonacid-fast bacteria (purple).
Mycobacterium Acid Fast Photos (Click on image to enlarge.)
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Application of Primary Stain: 1. Carbol fuchsin primary stain of acid-fat stain; 2. Carbol fuchsin being applied to slide that had been prepared with acid-fast controls and an unknown bacteria. Blotting paper has been put on top of the slide. Then the blotting paper is saturated with stain and heated over water bath; 3. Clothes pins are useful for handling the slide; 4. Blotting paper is discarded and slide is rinsed.
Application of Decolorizer: 1. Acid alcohol decolorizer for acid-fast stain; 2. Drizzle decolorizer down slide for 10 - 15 seconds, while watching to see that stain is removed from negative control; 3. Rinse.
Application of Counterstain: 1. Secondary stain (coounterstain), crystal violet; 2. Crystal violet is applied to slide and left for one minute; 3. Rinse; 4. Stained acid fast slide, with + control on left, unknown in center and - control on right. Go to > Video of Acid-fast Stain.
Non-acid-fast bacteria Staphylococcus epidermidis viewed under oil immersion @ 1000xTM
Photographic Guide to Completing the Acid-fast Stain
Double click on photo strip for a slideshow of larger images.
Photographic guides to differential stains
More Acid-fast Stain Resources